general stress protein, required for ribosome dimerization in the stationary phase, required for protection against paraquat stress
function
dimerization of ribosomes in the stationary phase, protection against paraquat stress
product
ribosome hibernation promoting factor
Genomic Context
categories
[category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)][category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress][category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]Gene
Coordinates
3,631,003 3,631,572
Phenotypes of a mutant
the mutant is cold-sensitivedelayed recovery from stationary phase and delayed [SW|germination] [Pubmed|26743942]The protein
Catalyzed reaction/ biological activity
[protein|search|Hpf ]binds the 30S subunits of hibernating [SW|ribosome]s, dimerization of [protein|search|Hpf ]results in 100S [SW|ribosome ]dimer formation [pubmed|28468753,26743942]Protein family
HPF/YfiA ribosome-associated protein family (single member, according to UniProt)Modification
phosphorylated on Arg-6 [Pubmed|22517742]Structure
[PDB|2YWQ] (N-terminal domain, from ''Thermus thermophilus'', 32% identity)[PDB|3K2T] (C-terminal domain, from ''Listeria monocytogenes'', 75% identity)[PDB|5NJT] (Hpf as part of the 100S [SW|ribosome]) [pubmed|28468753][SW|Localization]
cytoplasm (according to UniProt)Expression and Regulation
Operons
genes
[gene|4DBF4635B5C5A7541E8F97440D139DAA02547B99|yvyC]-[gene|C6012DEA301DF5B2DDD7E673A736BD3E5163F209|fliD]-[gene|4882FAD988FEDD0E72E4CAFB10C8DE61DDAC23B0|fliS]-[gene|4DE156D248FAF7DE3ED9AC35F006B5319A3C1BDA|fliT]-[gene|A6E7212D159F076F5D26F9C02F340B40C3667623|smiA]-[gene|06FEA5F205B40DD5CF8D9F1328E940D4543F4741|S1350]-[gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]
description
[pubmed|22383849]
sigma factors
[protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|26577401], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]view in new tabgenes
[gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]
description
[Pubmed|9852014]
sigma factors
[protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|9852014,15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon][protein|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH]: sigma factor, [Pubmed|9852014], in [regulon|DC3449D5F195E5C2E9E14FEC95396C8F1FDF73B4|SigH regulon]regulatory mechanism
[protein|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP]: activation, [Pubmed|25666134], in [regulon|638D6D2FCCA2A167EFED05CB4E60C2D78D5319AE|PhoP regulon]regulation
expressed in the stationary phase [Pubmed|26743942]view in new tabBiological materials
Mutant
BKE35310 ([gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAGAACGCCTCCCTT, downstream forward: _UP4_TAATGAAGAGAAGCCTTCCGBKK35310 ([gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35310 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCAAAGAACGCCTCCCTT, downstream forward: _UP4_TAATGAAGAGAAGCCTTCCGGP2598 ([gene|37B8B671B84266A7D65B90A9077A0DFF458D1851|hpf]::tet comIQ12L) (in DK1042) available in [SW|Jörg Stülke]'s labReferences
22517742,26743942,9852014,15805528,25666134,12107147,23663662,28468753,28468753,30619132